Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Decrease in lymphokine-activated killer sensitivity of a human renal-cell carcinoma cell line after cytokine

M Yanase1, T Tsukamoto, Y Kumamoto

  • 1Department of Urology, Sapporo Medical University, Japan.

Cancer Immunology, Immunotherapy : CII
|July 1, 1994
PubMed
Summary

Cytokine treatment decreased the sensitivity of renal-cell carcinoma cells to lymphokine-activated killer (LAK) cells. This reduction in LAK sensitivity was linked to protein synthesis, suggesting a novel therapeutic target.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification and isolation of common and tissue-specific geranylgeranylated gamma subunits of guanine-nucleotide-binding regulatory proteins in various tissues.

European journal of biochemistry·1992
Same author

Extra-adrenal pheochromocytoma-ganglioneuroma. A case report.

Pathology, research and practice·1992
Same author

Developmental regulation of cytochrome P-450 of intrasplenically transplanted fetal hepatocytes in spontaneously hypertensive rats.

Transplantation proceedings·1992
Same author

Manganese-containing superoxide dismutase in blood and urine during open-heart surgery.

Japanese circulation journal·1992
Same author

A Japanese pedigree of autosomal dominant congenital stationary night blindness with variable expressivity.

Ophthalmic paediatrics and genetics·1992
Same author

Effects of daily physical activity on insulin action in the elderly.

Journal of applied physiology (Bethesda, Md. : 1985)·1992

Area of Science:

  • Immunology
  • Oncology
  • Cell Biology

Background:

  • Cytokine therapy can modulate tumor cell sensitivity to immune effector cells like lymphokine-activated killer (LAK) cells.
  • The specific mechanisms underlying these modulations vary significantly between different tumor cell lines.

Purpose of the Study:

  • To investigate the impact of cytokine treatment on the LAK cell sensitivity of a human renal-cell carcinoma cell line (SMKT-R-3).
  • To elucidate the molecular mechanisms responsible for altered LAK sensitivity following cytokine exposure.

Main Methods:

  • Treatment of SMKT-R-3 cells with a combination of interferon gamma (IFN-γ) and tumor necrosis factor (TNF).
  • Assessment of LAK cell sensitivity and expression of intercellular adhesion molecule-1 (ICAM-1).
  • Evaluation of LAK sensitivity in the presence of actinomycin D, a protein synthesis inhibitor.

Related Experiment Videos

Main Results:

  • Cytokine treatment (IFN-γ and TNF) decreased the LAK sensitivity of SMKT-R-3 cells.
  • Cytokine treatment led to increased expression of ICAM-1 on the tumor cell surface.
  • Inhibition of protein synthesis with actinomycin D restored LAK sensitivity to baseline levels.

Conclusions:

  • The observed decrease in LAK sensitivity of renal-cell carcinoma cells by cytokine treatment is mediated by protein synthesis.
  • These findings suggest that protein synthesis occurring during LAK cell interaction with tumor cells plays a crucial role in modulating immune response.