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Generation and molecular analyses of two rheumatoid synovial fluid-derived IgG rheumatoid factors
Arthritis and Rheumatism
|July 1, 1993
Objective:
To study the Ig genes that encode IgG rheumatoid factor (IgG-RF) from rheumatoid synovial fluid.
Methods:
We used rheumatoid synovial fluid B cells to generate IgG-RF-secreting hybridomas. We then characterized their binding properties and determined their nucleotide sequences.
Results:
Two monospecific IgG-RFs were obtained. Sequence analysis of the RFs revealed a new V lambda gene family (designated V lambda 9) and extensive somatic diversification, including a duplication-insertion of 18 nucleotides (6 amino acid residues) into a hypervariable region.
Conclusion:
The data provide further support for an antigen-driven response in the sustained production of potentially pathogenic IgG-RFs in rheumatoid synovium.