Structure of the Pisum sativum seed lipoxygenase gene lox1:Ps:3

We have isolated the Pisum sativum (pea) lox1:Ps:3 gene which encodes a lipoxygenase that is 84% identical in coding sequence to the LOX-3 gene from Glycine max (soybean). The 10028 bp sequence includes 5895 bp 5' to the transcription start site and 707 bp 3' to the stop codon. The coding region of lox1:Ps:3 contains eight introns, at positions equivalent to those in the soybean LOX-3 and L-4, the Phaseolus vulgaris (Frenchbean) LOX1, and the Pisum sativum lox1:Ps:2 genes. The first intron is unusually small. The 5'-flanking sequence contains two regions that have the potential to form hairpin structures, but few motifs known to interact with transcription factors. Despite their similarity in terms of expression, the lox1:Ps:3 and lox1:Ps:2 genes have little similarity within their 5'-flanking regions.