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Acrolein fixation for immunostaining tyrosine-hydroxylase in paraffin sections
1Center of Endocrine Studies, School of Medicine, National University of La Plata, Argentina.
Abstract:
Routine histological fixatives barely preserve tyrosine-hydroxylase immunoreactivity in paraffin sections. fixation in 5% acrolein in phosphate buffer, pH 7.4, resulted in good preservation of the enzyme in the tissues investigated.
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