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A monoclonal antibody specific for a 40 kDa polypeptide recognizes pathogenic strains of Entamoeba histolytica
1Department of Genetics and Molecular Biology, Centro de Investigación y de Estudios Avanzados del IPN, Mexico City, Mexico.
Archives of Medical Research
|January 1, 1994
Abstract:
In vitro interaction of Entamoeba histolytica trophozoites with collagen induces the intracellular formation and release of electron-dense granules (EDGs). We determined that four polypeptides in EDGs total antigen by SDS-PAGE were absent in trophozoite extracts. A monoclonal antibody raised against these EDGs recognized a polypeptide of 40 kDa specific for the pathogenic strains of E. histolytica. In addition, this mAb recognized a 96 kDa peptide masked by carbohydrates.