Related Experiment Videos
Polymerase chain reaction based cDNA cloning of wheat profilin: a potential plant allergen
H P Rihs1, P Rozynek, K May-Taube
1Abteilung Molekulare Genetik, Berufsgenossenschaftliches, Forschungsinstitut für Arbeitsmedizin, Ruhr-Universität, Bochum, Deutschland.
International Archives of Allergy and Immunology
|October 1, 1994
Abstract:
Highly degenerate profilin-specific primers were used to amplify by polymerase chain reaction the profilin-coding sequence of wheat cDNA. Analyzing the sequencing data after subcloning revealed the existence of three isoforms. The high amino acid sequence identity with the birch pollen allergen Betv II suggests that wheat profilin may be a potential plant allergen, relevant in food allergy.