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DNA variability and parentage testing in captive Waldrapp ibises

E N Signer1, C R Schmidt, A J Jeffreys

  • 1Department of Genetics, University of Leicester, England.

Molecular Ecology
|August 1, 1994
PubMed
Summary
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DNA fingerprinting using minisatellite probes effectively identified individual Waldrapp ibises and their parentage. This technology is crucial for managing genetic diversity in captive breeding programs for this endangered species.

Area of Science:

  • Conservation Genetics
  • Avian Biology
  • Molecular Ecology

Background:

  • The Waldrapp ibis (Geronticus eremita) is critically endangered, with captive breeding as a primary survival strategy.
  • Maintaining genetic diversity is essential for viable populations in conservation programs.
  • Accurate individual identification and pedigree tracking are vital for effective selective breeding.

Purpose of the Study:

  • To apply DNA fingerprinting technology for individual identification and parentage verification in captive Waldrapp ibises.
  • To assess the utility of minisatellite probes for monitoring population variability and completing pedigree records.
  • To evaluate the accuracy of DNA-based parentage assignment against observed breeding behavior.

Main Methods:

  • DNA fingerprinting was performed on 39 captive Waldrapp ibises using five minisatellite probes (MS1, MS31, p lambda g3, pCMS12, MS51).

Related Experiment Videos

  • Probes were analyzed for their ability to detect polymorphic loci for individual identification and parentage resolution.
  • A sex-determining marker was identified using probes MS31 and p lambda g3.

    • Main Results:

    • Highly polymorphic banding patterns were obtained, enabling individual identification and parentage resolution.
    • Probe MS51 was locus-specific with six alleles; MS31 and p lambda g3 identified sex.
    • Parentage was unambiguously assigned for most individuals, with DNA data revealing stud book inaccuracies.
    • DNA analysis identified interchanges of individuals, correcting stud book records.

    Conclusions:

    • Minisatellite DNA fingerprinting provides a sensitive and reliable method for individual identification and pedigree verification in captive Waldrapp ibises.
    • This technology is instrumental in managing genetic diversity and ensuring the long-term viability of endangered species through captive breeding.
    • The findings highlight the importance of molecular tools in complementing traditional breeding management and ensuring accurate population records.