Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Typing for MLC determinants: methods and applications

M Thomsen, L P Ryder, A Svejgaard

    Scandinavian Journal of Immunology
    |June 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The HLA-DP2 protein binds the immunodominant epitope from myelin basic protein, MBP85-99, with high affinity.

    Tissue antigens·2011
    Same author

    Neonatal Immune Thrombocytopenia Due to Allo-or Autoantibodies: Clinical and Immunological Analysis of 83 Cases.

    Platelets·2010
    Same author

    Nomenclature for factors of the HLA system, 2010.

    Tissue antigens·2010
    Same author

    An update to HLA nomenclature, 2010.

    Bone marrow transplantation·2010
    Same author

    Identification of new sensitive biomarkers for the in vivo response to interferon-beta treatment in multiple sclerosis using DNA-array evaluation.

    European journal of neurology·2009
    Same author

    Gene expression analysis of interferon-beta treatment in multiple sclerosis.

    Multiple sclerosis (Houndmills, Basingstoke, England)·2008
    Same journal

    Recent Thymic Emigrant Levels in Inborn Errors of Immunity: Is Their Diagnostic Value Greater Than We Think?

    Scandinavian journal of immunology·2026
    Same journal

    The EBNA-1 Conundrum: Does Epstein-Barr Virus Invoke Autoimmune Pathology in a Population Subset by Poorly Purine-Loading Its Major Latency-Maintaining Transcript?

    Scandinavian journal of immunology·2026
    Same journal

    Unsupervised Flow Cytometry Reveals a Constant Shift Towards Activated CD4<sup>+</sup> T Cell Subsets in APECED.

    Scandinavian journal of immunology·2026
    Same journal

    RETRACTION: Interleukin-13 Induces T Helper Type 2 Immune Responses in OVA-Immunized BALB/c Mice Bearing a T Cell Lymphoma.

    Scandinavian journal of immunology·2026
    Same journal

    Development of a Detection Method for Soluble Urokinase-Type Plasminogen Activator Receptor and Its Application in Predicting Prognosis of Severe COVID-19.

    Scandinavian journal of immunology·2026
    Same journal

    Crohn's Disease Enteritis: Pathophysiological Mechanisms and Therapeutic Approaches.

    Scandinavian journal of immunology·2026
    See all related articles

    MLC typing using HLA-D homozygous cells and primed lymphocyte typing (PLT) effectively identify HLA-D specificities. These methods show excellent correlation for clinical applications like transplantation and disease association studies.

    Area of Science:

    • Immunogenetics
    • Cellular immunology

    Background:

    • Mixed lymphocyte culture (MLC) typing is crucial for assessing human leukocyte antigen (HLA) compatibility.
    • Established methods rely on a panel of inactivated HLA-D homozygous cells to determine HLA-D specificities.

    Purpose of the Study:

    • To evaluate the efficacy of MLC typing using homozygous cells and primed lymphocyte typing (PLT).
    • To explore the correlation between these typing methods and their clinical applications.

    Main Methods:

    • Utilized inactivated HLA-D homozygous cells for MLC typing.
    • Employed primed lymphocyte typing (PLT) involving secondary MLC cultures.
    • Analyzed results considering both responder and stimulator cell capacities, with a focus on the 75th percentile evaluation.

    Related Experiment Videos

    Main Results:

    • Demonstrated an excellent correlation between MLC typing and PLT.
    • Confirmed the reliability of these methods when using well-characterized, HLA-D homozygous priming cells.
    • Identified clinical applications in transplantation and HLA-disease associations.

    Conclusions:

    • MLC typing and PLT are reliable methods for determining HLA-D specificities.
    • These immunogenetic techniques have significant clinical relevance in transplantation and disease research.