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Evidence for an intermediate in the enzymatic formation of uroporphyrinogen III
C Pichon1, B P Atshaves, N J Stolowich
1Department of Chemistry, Texas A&M University, College Station 77843-3255.
Bioorganic & Medicinal Chemistry
|March 1, 1994
Abstract:
Evidence for an azafulvene intermediate in the enzymatic formation of Uroporphyrinogen III has been obtained. Using conditions to slow down the enzyme activity (high pH, low temperature), the transient species was trapped with ammonium ions as aminomethylbilane and with sodium borohydride as methylbilane, and observed by 13C-NMR.