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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up
Published on: March 26, 2018
[Lymphocyte subpopulations determined by flow cytometry in chronic type B lymphatic leukemia]
J Kvasnicka1, P Kruzík, E Cmunt
1Oddĕlení klinické hematologie, VFN, Praha.
Casopis Lekaru Ceskych
|August 22, 1994
Summary
Flow cytometry analysis reveals distinct surface markers on lymphocytes in chronic lymphatic leukemia type B (B-CLL) patients. Elevated CD19+ CD5+ (B1) cells and decreased CD5- B2 cells are key diagnostic indicators for B-CLL.
Area of Science:
- Immunology
- Hematology
Context:
- Flow cytometry is crucial for analyzing lymphocyte surface markers.
- Accurate immunophenotyping aids in understanding cell populations.
Purpose:
- To detect surface markers on lymphocytes in peripheral blood of patients with lymphatic leukemia using flow cytometry.
- To compare these markers with those in healthy individuals.
Summary:
- Flow cytometry identified significantly higher CD19+ CD5+ (B1) lymphocytes (83.4%) in B-CLL patients compared to controls (3.5%).
- B-CLL patients showed decreased B2 (CD5-) lymphocytes (2.8% vs 9.0% in controls) and altered T-cell subsets (CD4+ CD45RA+ and CD8+ CD11b+).
- These immunophenotypic changes correlate with B-CLL diagnosis, autoimmune complications, and hypogammaglobulinemia.
Impact:
- Flow cytometry with double fluorescence aids in accurate B-CLL diagnosis.
- This method assists in monitoring therapeutic efficacy.
- It enhances understanding of the immunopathology underlying B-CLL.
