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Specific receptor for vasoactive intestinal contractor in myenteric cholinergic neurones
1Department of Pharmacology II, Nagasaki University School of Medicine, Japan.
European Journal of Pharmacology
|June 2, 1994
Summary
Vasoactive intestinal contractor (VIC) stimulates acetylcholine release from guinea-pig ileum neurons. The VIC receptor is located on cholinergic neurons, not smooth muscle cells, indicating specific neuronal targeting.
Area of Science:
- Neurogastroenterology
- Pharmacology
- Cellular Biology
Background:
- Vasoactive intestinal contractor (VIC) is implicated in regulating acetylcholine release.
- Understanding the specific receptor location for VIC is crucial for its pharmacological targeting.
Purpose of the Study:
- To investigate the receptor for vasoactive intestinal contractor (VIC) and its role in acetylcholine release in the guinea-pig ileum.
- To determine if VIC interacts with endothelin receptors.
Main Methods:
- Experiments were conducted on guinea-pig ileum strips preloaded with [3H]choline.
- Tritium outflow and muscle contractions were measured in response to VIC.
- Desensitization studies with endothelin-1, -2, and -3 were performed.
Main Results:
- VIC evoked a concentration-dependent and Ca(2+)-dependent tritium outflow, sensitive to tetrodotoxin.
- VIC-induced contractions showed both tetrodotoxin-sensitive and -resistant components.
- No cross-desensitization was observed between VIC and endothelins for tritium outflow.
- Tetrodotoxin-resistant contractions were abolished after endothelin desensitization.
Conclusions:
- The VIC receptor is located on cholinergic neurons, mediating acetylcholine release.
- VIC does not share cross-desensitization with endothelin receptors in this context.
- VIC-specific receptors are not located on guinea-pig ileum smooth muscle cells.