Related Experiment Video
Updated: Sep 21, 2026

Measurement of Heme Synthesis Levels in Mammalian Cells
Published on: July 9, 2015
Engineering Ascaris hemoglobin oxygen affinity in sperm whale myoglobin: role of tyrosine B10
C Travaglini Allocatelli1, F Cutruzzolà, A Brancaccio
1Department of Biochemical Sciences A Rossi Fanelli, University of Rome La Sapienza, Italy.
Abstract:
The contribution to oxygen stabilization of a tyrosine residue in topological position (B10) has been studied in sperm whale myoglobin by simultaneous replacement of residues at positions (B10), (E7) and (E10) as suggested by analysis of the sequence of high oxygen affinity hemoglobins, such as that of the nematode Ascaris suum. Kinetic and equilibrium experiments with the gaseous ligands oxygen and carbon monoxide show that indeed the introduction of tyrosine (B10), together with replacement of the distal histidine (E7) with glutamine, is associated with a large decrease in the oxygen dissociation rate constant. Our results are consistent with the possible formation in the distal pocket of two hydrogen bonds with the iron-bound oxygen.
More Related Videos
11:48Development of a Colloidal Gold-based Immunochromatographic Test Strip for Detection of Cetacean Myoglobin
Published on: July 13, 2016
08:51Intra-cardiac Side-Firing Light Catheter for Monitoring Cellular Metabolism using Transmural Absorbance Spectroscopy of Perfused Mammalian Hearts
Published on: May 12, 2019
Related Concept Videos
Gene Families
Occasionally these regions can be adapted to take on new roles within the organism, becoming novel genes...
Protein Families
ATP Synthase: Mechanism
Globular Proteins
Globular proteins serve many important physiological functions, such as acting as enzymes, cellular messengers, and molecular transporters. These roles often require the proteins to be soluble in the aqueous...
Hemoglobin
When all four heme groups are bound to oxygen, the resulting molecule is called oxyhemoglobin. As a result, arterial blood...
Oxygen Transport in the Blood