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Related Experiment Videos

Long circulating, cationic liposomes containing amino-PEG-phosphatidylethanolamine

S Zalipsky1, E Brandeis, M S Newman

  • 1Liposome Technology Inc., Menlo Park, CA 94025.

FEBS Letters
|October 10, 1994
PubMed
Summary

Positively charged liposomes using Amino-polyethylene glycol-phosphatidylethanolamine (AminoPEG-PE) show comparable in vivo circulation and biodistribution to standard methoxy-PEG-PE liposomes, impacting drug delivery strategies.

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Area of Science:

  • Biomedical Engineering
  • Drug Delivery Systems
  • Nanotechnology

Background:

  • Attaching ligands to polyethylene glycol (PEG) grafted liposomes is crucial for targeted drug delivery.
  • The impact of positively charged groups on PEG-liposome interactions in vivo remains largely unexplored.
  • Amino-polyethylene glycol-phosphatidylethanolamine (AminoPEG-PE) offers a potential route for creating cationic liposomes.

Purpose of the Study:

  • To investigate the in vivo behavior of liposomes functionalized with AminoPEG-PE.
  • To determine the influence of cationic surface charges on liposome circulation time and biodistribution.
  • To assess the potential of AminoPEG-PE modified liposomes for targeted delivery applications.

Main Methods:

  • Synthesis of AminoPEG-PE from alpha-amino-omega-hydroxy-PEG through a four-step process.

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  • Preparation of liposomes incorporating AminoPEG-PE.
  • Evaluation of liposome blood circulation and biodistribution in vivo.
  • Comparison with liposomes prepared using standard methoxy-PEG-PE.

    • Main Results:

    • Liposomes functionalized with AminoPEG-PE exhibited cationic surface properties due to the presence of amine groups.
    • Despite the cationic nature, these liposomes demonstrated in vivo circulation and biodistribution profiles comparable to conventional methoxy-PEG-PE liposomes.
    • No significant adverse effects on liposome stability or clearance were observed due to the cationic charge.

    Conclusions:

    • AminoPEG-PE can be effectively used to create cationic liposomes without compromising their long-circulating properties.
    • The findings suggest that cationic liposomes may be suitable for systemic delivery applications, including the delivery of lipidic-polynucleotide complexes.
    • This research opens avenues for advanced targeted therapies utilizing modified liposome formulations.