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The mouse type-III procollagen-encoding gene: genomic cloning and complete DNA sequence
1Department of Molecular Genetics, University of Texas M.D. Anderson Cancer Center, Houston 77030.
Gene
|September 30, 1994
Summary
Researchers sequenced the mouse type-III collagen gene (mCol3), detailing its structure and identifying optimal sequencing methods. The mCol3 gene spans 37.6 kb and contains 51 exons, showing high similarity to human type-III collagen.
Area of Science:
- Genomics
- Molecular Biology
- Biochemistry
Background:
- Type-III collagen is a fibrillar collagen crucial for tissue structure.
- Understanding the genetic basis of collagen production is vital for research into connective tissue disorders.
Purpose of the Study:
- To characterize the entire mouse type-III collagen gene (mCol3) locus.
- To determine optimal sequencing strategies for large genes.
- To compare the mouse mCol3 gene with its human counterpart.
Main Methods:
- Isolation and characterization of overlapping cosmid clones covering the mCol3 locus.
- Restriction mapping and sequencing of 43.6 kb of the gene and flanking regions.
- Evaluation of random versus directed sequencing ratios for efficiency.
Main Results:
- The mCol3 gene spans 37.6 kb from the transcription start point to the polyadenylation site.
- The gene comprises 51 exons and contains repetitive elements.
- Predicted mCOL3 mRNA is 4767 nt and the polypeptide is 1464 amino acids, with 91% amino acid identity to human COL3.
Conclusions:
- The study provides a comprehensive characterization of the mouse type-III collagen gene.
- A cost-efficient sequencing method involving twofold redundancy was identified.
- The high sequence identity suggests conserved function between mouse and human type-III collagen.