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Related Experiment Videos

A method for labeling cells for positron emission tomography (PET) studies

R J Melder1, D Elmaleh, A L Brownell

  • 1Department of Radiation Oncology, Harvard Medical School, Boston, MA.

Journal of Immunological Methods
|September 30, 1994
PubMed
Summary

Protein labeling with carbon-11 methyl iodide (MI) offers a stable method for tracking cells using positron emission tomography (PET). This technique allows for practical quantification of cell distribution in vivo without affecting cell viability.

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Area of Science:

  • Nuclear Medicine
  • Immunology
  • Biotechnology

Background:

  • Positron emission tomography (PET) offers high-resolution 3D imaging for monitoring cell delivery to tissues.
  • Developing rapid and stable cell labeling methods is crucial for effective PET imaging.
  • Current methods include metabolic incorporation of 2-[18F]fluorodeoxyglucose (2-[18F]FDG) or protein labeling.

Purpose of the Study:

  • To evaluate and compare two cell labeling methods for PET imaging: 2-[18F]FDG and carbon-11 methyl iodide (MI).
  • To assess the stability, cell viability, and functional activity of labeled cells.
  • To demonstrate the feasibility of tracking labeled cells in vivo using PET.

Main Methods:

  • Expanded IL-2 activated mouse natural killer lymphocytes in culture.

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  • Labeled cells using either 2-[18F]FDG (metabolic) or [11C]MI (protein labeling).
  • Assessed label uptake, stability, cell viability, and cytotoxic activity; injected labeled cells into mice for PET imaging.

    • Main Results:

    • 2-[18F]FDG labeling was rapid but showed significant decay (21% loss in 1 hour).
    • [11C]MI labeling demonstrated stable association for 60 minutes without compromising cell viability or function.
    • PET imaging of [11C]MI-labeled cells in mice showed lung accumulation consistent with microscopy.

    Conclusions:

    • [11C]MI labeling provides a stable and practical method for tracking lymphocytes using PET.
    • This technique enables rapid quantification of systemic cell distribution.
    • PET imaging with MI-labeled cells is a promising tool for monitoring cell delivery in vivo.