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Crystallization and preliminary X-ray diffraction analysis of the ArsC protein from the Escherichia coli arsenical
V S de Mel1, M A Doyle, T B Gladysheva
1Department of Biochemistry, Wayne State University, School of Medicine, Detroit, MI 48201.
Journal of Molecular Biology
|October 7, 1994
Abstract:
Diffraction data to 3.0 A resolution were collected on crystals of ArsC protein from the conjugative resistance factor R773 which mediates arsenical resistance in the Gram negative bacterium Escherichia coli. The crystal system is tetragonal, a = 116.2 A, c = 145.0 A and the space group is either P4(1)2(1)2 or P4(3)2(1)2. The most probable range for the contents of the asymmetric unit is four to eight ArsC molecules (M(r) = 15,811).