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Updated: Aug 9, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Mycoflora, and mycotoxins production in Nigerian corn and corn-based snacks
L O Adebajo1, A A Idowu, O O Adesanya
1Department of Biological Sciences, Ogun State University, Ago-Iwoye, Nigeria.
Abstract:
Thirty-one fungal species, mostly toxigenic and belonging to 11 genera were isolated from corn, corn cake and corn roll snack samples. Aspergillus, Penicillium and Fusarium accounted for 10, 6 and 3 of the species and altogether, they constituted 90, 94 and 88 percent of the total fungi in corn, corn cake and corn roll snack respectively. Mycotoxins (aflatoxins and ochratoxin A) were detected in 45, 80 and 12 percent while the means and ranges of the total aflatoxins recorded were: 200(25-770 ppb); 233(15-1070 ppb) and 55(10-160 ppb) for corn, corn cake and corn roll snack samples respectively. Ochratoxin A was detected at toxicologically significant levels in only 15 percent of the corn cake samples analyzed. All the strains of Aspergillus flavus and A. ochraceus tested produced aflatoxin B and ochratoxin A, respectively, when they were cultured on each of the three substrates. In each case, substantial quantities of the toxins were produced from 25 to 35 degrees C with the peak level recorded at 30 degrees C. Toxin production was detected only in substrates with 15 percent moisture content and above; reaching the maximum at 25 or 30 percent moisture level. No substantial differences in the amount of toxins were elaborated with further increase in substrates' moisture content. Of the three substrates, corn cake was the most suitable for aflatoxin B production while they were all equally suitable for the elaboration of ochratoxin A.
Insights
Toxigenic fungi and mycotoxins like aflatoxins were prevalent in corn products. Corn cake showed the highest aflatoxin levels, with optimal production at 30°C and high moisture content.
Area of Science:
- Food Science
- Mycology
- Food Safety
Background:
- Fungal contamination and mycotoxin production in food commodities pose significant health risks.
- Corn and its processed products are susceptible to contamination by toxigenic fungi.
Purpose of the Study:
- To identify fungal species and quantify mycotoxin contamination in corn, corn cake, and corn roll snacks.
- To investigate the influence of temperature and moisture on mycotoxin production by specific fungal strains.
Main Methods:
- Isolation and identification of fungal species from various corn-based food samples.
- Quantification of aflatoxins and ochratoxin A using analytical methods.
- Controlled laboratory experiments to assess mycotoxin production under varying temperature and moisture conditions.
Main Results:
- Thirty-one fungal species were isolated, with Aspergillus, Penicillium, and Fusarium dominating.
- Mycotoxins were detected in 45% (corn), 80% (corn cake), and 12% (corn roll snack) of samples.
- Optimal aflatoxin B and ochratoxin A production occurred at 30°C and moisture levels of 25-30%.
Conclusions:
- Corn-based food products can be significant sources of fungal contamination and mycotoxins.
- Corn cake is particularly susceptible to aflatoxin contamination.
- Environmental factors like temperature and moisture critically influence mycotoxin biosynthesis in fungi.
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