Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple procedure for optimising the polymerase chain reaction (PCR) using modified Taguchi methods

B D Cobb1, J M Clarkson

  • 1Microbial Pathogenicity Group, School of Biology and Biochemistry, University of Bath, UK.

Nucleic Acids Research
|September 11, 1994
PubMed
Summary

Taguchi methods efficiently optimize Polymerase Chain Reaction (PCR) by revealing component effects and interactions with minimal experiments. This approach enhances product yield and controls Random Amplified Polymorphic DNA (RAPD-PCR) profiles.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Striving for humane deaths for laboratory mice: hypobaric hypoxia provides a potential alternative to carbon dioxide exposure.

Proceedings. Biological sciences·2023
Same author

Somaclonal variation in watercress for resistance to crook root disease.

Plant cell reports·2013
Same author

Trehalose-hydrolysing enzymes of Metarhizium anisopliae and their role in pathogenesis of the tobacco hornworm, Manduca sexta.

Journal of invertebrate pathology·2002
Same author

Acid phosphatases of Metarhizium anisopliae during infection of the tobacco hornworm Manduca sexta.

Archives of microbiology·2001
Same author

Single tube genotyping of sickle cell anaemia using PCR-based SNP analysis.

Nucleic acids research·2001
Same author

Characterization of SNP1, a cell wall-degrading trypsin, produced during infection by Stagonospora nodorum.

Molecular plant-microbe interactions : MPMI·2000

Area of Science:

  • Molecular Biology
  • Biotechnology
  • Process Optimization

Background:

  • Taguchi methods are established for industrial process design.
  • Polymerase Chain Reaction (PCR) optimization traditionally requires extensive experimentation.
  • Understanding reaction component interactions is crucial for PCR efficiency.

Purpose of the Study:

  • To evaluate the suitability of Taguchi methods for optimizing PCR.
  • To demonstrate a more efficient approach to PCR optimization compared to conventional strategies.
  • To apply Taguchi methods for qualitative optimization of Random Amplified Polymorphic DNA (RAPD-PCR) profiles.

Main Methods:

  • Application of Taguchi methods for experimental design in PCR.
  • Simultaneous analysis of reaction component effects and interactions.

Related Experiment Videos

  • Utilizing a reduced number of reactions for optimization.
  • Qualitative investigation of RAPD-PCR profiles.

    • Main Results:

    • Taguchi methods identified key reaction components affecting PCR product yield.
    • The technique revealed significant interactions between PCR components.
    • Optimization of RAPD-PCR product size and distribution was achieved.
    • Reduced experimental effort was required compared to conventional methods.

    Conclusions:

    • Taguchi methods offer an efficient and effective strategy for PCR optimization.
    • This approach simplifies the determination of critical reaction parameters.
    • Taguchi methods are applicable to both quantitative yield and qualitative profile optimization in PCR-based techniques.