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Rapid differentiation of avian Haemophilus-like strains by their whole-cell carbohydrate patterns
M Mouahid1, R Mutters, L A Devriese
1Institute of Medical Microbiology, Philipps University, Marburg, Germany.
Veterinary Microbiology
|June 1, 1994
Abstract:
The whole-cell carbohydrate patterns of 14 Haemophilus-like strains isolated from diseased birds were examined by capillary gas chromatography/mass spectrometry. The analysis of the peracetylated aldononitrile and O-methyloxime derivatives allowed the differentiation between the phenotypically and genetically different isolates. Starting from a pure culture the procedure needs only 5 hours for the preparation of the samples and 30 minutes for subsequent analysis and is of special value for rapid diagnosis.