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Imaging reactive oxygen species in asthma
I Vachier1, C Le Doucen, J Loubatière
1INSERM Unité 58, Montpellier, France.
Journal of Bioluminescence and Chemiluminescence
|May 1, 1994
Summary
Asthma patients show increased reactive oxygen species release from inflammatory cells like mononuclear phagocytes. These cells are primed in vivo and more easily stimulated, indicating altered immune responses in asthma.
Area of Science:
- Immunology
- Respiratory Medicine
- Cell Biology
Background:
- Asthma involves inflammatory cell infiltration, particularly mononuclear phagocytes.
- Mononuclear phagocytes (alveolar macrophages, blood monocytes) in asthmatics release more reactive oxygen species (ROS) than in healthy individuals.
Purpose of the Study:
- To measure superoxide anion and other ROS in inflammatory cells from asthmatic patients.
- To investigate the activation status of inflammatory cells in asthma using chemiluminescence assays.
Main Methods:
- Chemiluminescence assays using a luminometer to analyze cells in suspension.
- Chemiluminescence assays with a video-imaging camera for adherent cells, stimulated with PMA and/or IgE/anti-IgE.
Main Results:
- Both luminometer and video-imaging techniques demonstrated that inflammatory cells (alveolar macrophages, blood monocytes, PMN, lymphocytes) are primed in vivo in asthmatic patients.
- These cells showed enhanced stimulation responses in asthma compared to healthy subjects.
Conclusions:
- Inflammatory cells in asthmatic patients exhibit spontaneous in vivo priming.
- In vitro analysis of adherent cells provides insights into their in vivo physiological condition in asthma.