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Related Experiment Videos

Modulation of leukocyte migration in mesenteric interstitium

K Bienvenu1, N Harris, D N Granger

  • 1Department of Physiology and Biophysics, Louisiana State University Medical Center, Shreveport 71130.

The American Journal of Physiology
|October 1, 1994
PubMed
Summary

N-formylmethionyl-leucyl-phenylalanine (FMLP) significantly enhances leukocyte migration in rat mesentery interstitium more than other inflammatory stimuli. This study introduces a novel in vivo model for studying extravascular leukocyte movement.

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Area of Science:

  • Immunology
  • Cell Biology
  • Physiology

Background:

  • Leukocyte migration is crucial in inflammatory responses.
  • Understanding extravascular leukocyte movement is vital for disease research.
  • Existing models have limitations in studying interstitial leukocyte dynamics.

Purpose of the Study:

  • To investigate leukocyte migration rates in the rat mesenteric interstitium.
  • To compare the effects of various inflammatory stimuli on leukocyte migration.
  • To establish a novel in vivo model for studying extravascular leukocyte dynamics.

Main Methods:

  • Intravital videomicroscopy was employed to observe leukocyte migration.
  • The rat mesentery was exposed to N-formylmethionyl-leucyl-phenylalanine (FMLP), leukotriene B4 (LTB4), platelet-activating factor (PAF), or ischemia-reperfusion (I-R).

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  • The impact of receptor antagonists and enzyme inhibitors on migration was assessed.

    • Main Results:

    • All tested inflammatory stimuli increased interstitial leukocyte migration rates compared to controls.
    • FMLP stimulation resulted in significantly higher migration rates than LTB4, PAF, or I-R.
    • Ischemia-reperfusion-induced migration was not affected by PAF- and LTB4-receptor antagonists.
    • FMLP-stimulated migration was unaffected by elastase/cathepsin G inhibitors, anti-CD11/CD18 antibody, or altered interstitial hydration.

    Conclusions:

    • FMLP is a potent stimulator of leukocyte migration in the mesenteric interstitium.
    • LTB4 and PAF are unlikely mediators of ischemia-reperfusion-induced leukocyte migration.
    • The developed in vivo model is effective for studying factors influencing extravascular leukocyte migration.