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[Rapid determination of oleanolic acid in tablets by HPLC]
Summary
This study details a High-Performance Liquid Chromatography (HPLC) method for precise sample analysis. The validated method achieves a low limit of determination and high precision, suitable for quantitative measurements.
Area of Science:
- Analytical Chemistry
- Chromatography
Context:
- High-Performance Liquid Chromatography (HPLC) is a crucial technique in analytical chemistry.
- Method development and validation are essential for reliable quantitative analysis.
Purpose:
- To develop and validate an HPLC method for sample analysis.
- To establish the method's sensitivity and precision.
Summary:
- The HPLC analysis utilized an ultrasphere-ODS column with a methanol-water mobile phase.
- The method demonstrated a lowest limit of determination of 0.4 micrograms.
- A relative standard deviation of 2.41% was achieved across different sample concentrations.
Impact:
- Provides a validated HPLC method with high sensitivity and precision.
- Contributes to the advancement of quantitative analytical techniques.
- Supports accurate chemical analysis in various scientific fields.