Effects of preservation and sterilization on cortical bone grafts. A scanning electron microscopic study

The effects of preservation and sterilization on the structural properties of cortical bone were investigated. Specimens of cortical bone from rat tibiae were frozen (-70 degrees C for 28 days), freeze-dried, irradiated (1, 5, 25 and 50 kGy) or autoclaved (at 134 degrees C for 3 or 5 min), and examined by scanning electron microscopy. Cryopreservation and irradiation had no deleterious effects on the surface structure of the cortical bone. Freeze-drying caused microcracks running parallel to the mineralized fiber bundles. After autoclaving, a time-dependent distension, swelling and amalgamation of the fibrillary matrix was observed. This denaturation of the organic matrix was more pronounced after 5 min than 3 min autoclaving. The alterations of the fibrillary structure described above might be due to a preservation- and sterilization-induced decrease of the biological and biomechanical potential of bone grafts.