Regulation of alpha 1b-adrenergic receptor gene expression in rat liver cell lines

alpha 1b-Adrenergic receptor gene expression was investigated in two rat hepatic cell lines, Clone 9 and McA-RH7777 cells. By Northern blot analysis, Clone 9 cells expressed a 2.7 kb alpha 1b-adrenergic receptor gene transcript whereas two transcripts, 3.3 kb and 2.7 kb, were observed in total cellular RNA isolated from rat liver. A binding site for the alpha 1-adrenergic antagonist [3H]prazosin was observed in Clone 9 cell membrane preparations (Bmax = 47 +/- 7 fmol/mg protein and Kd = 0.11 +/- 0.02 nM, n = 5). In contrast, alpha 1b-adrenergic receptor gene transcripts could not be detected in total cellular RNA prepared from McA-RH7777 cells by either Northern blot analysis or ribonuclease protection assays. However, results from nuclear run-off assays indicated that the alpha 1b-adrenergic receptor gene was transcribed in McA-RH7777 cells and alpha 1b-adrenergic receptor gene transcripts were observed in McA-RH7777 cell nuclear RNA. These results suggest that alpha 1b-adrenergic receptor gene expression in liver may be regulated in part post-transcriptionally and that this level of regulation may be altered or disrupted in the Clone 9 and McA-RH7777 cell lines.