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Effect of partial hepatectomy on lactic dehydrogenase-5 clearance in mice
Abstract:
The capacity of lactic dehydrogenase-5 (LDH-5) clearance of the liver following partial hepatectomy was investigated in ICR mice. Compared with sham-operated mice, the LDH-5 clearance rate was decreased slightly after the removal of 30 or 50%. Removal of approximately 65% of the liver induced a statistically significant decrease in the clearance rate. LDH activity in the blood was increased by the removal of about 50 and 65%, but not 30% of the liver. These results suggest that increase in LDH activity after hepatectomy may be due to decreased catabolic activity of the liver and the liver may play a significant role in the catabolism of LDH-5. The LDH-5 clearance capacity of the liver will be discussed.
Insights
The liver
Area of Science:
- Hepatology and biochemistry research focusing on liver function and enzyme metabolism.
Background:
- Partial hepatectomy, the surgical removal of liver tissue, triggers adaptive responses in the remaining liver.
- Lactate dehydrogenase (LDH) is an enzyme found in various tissues, and its isoenzymes, like LDH-5, can indicate cellular damage or metabolic state.
Purpose of the Study:
- To investigate the impact of partial hepatectomy on the liver's capacity to clear lactate dehydrogenase-5 (LDH-5).
- To determine the relationship between the extent of liver resection and changes in LDH-5 clearance and blood activity.
Main Methods:
- ICR mice underwent partial hepatectomy, with varying percentages of liver tissue (30%, 50%, 65%) removed.
- Sham-operated mice served as controls.
- LDH-5 clearance rates and blood LDH activity were measured and compared between groups.
Main Results:
- A slight decrease in LDH-5 clearance was observed after 30% or 50% liver removal.
- A statistically significant decrease in LDH-5 clearance occurred following 65% liver resection.
- Blood LDH activity increased significantly after 50% and 65% hepatectomy, but not after 30% removal.
Conclusions:
- The liver plays a crucial role in the catabolism of LDH-5.
- Reduced LDH-5 clearance after significant hepatectomy suggests impaired hepatic catabolic activity.
- Elevated blood LDH levels post-hepatectomy may result from decreased liver clearance capacity.