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Updated: Oct 13, 2026

Characterization of Complex Systems Using the Design of Experiments Approach: Transient Protein Expression in Tobacco as a Case Study
Published on: January 31, 2014
Tissue-specific expression of an oat 12S seed globulin gene in developing tobacco seeds: differential mRNA and
R Schubert1, R Panitz, R Manteuffel
1Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany.
Abstract:
We studied the expression of the oat globulin gene asglo5 in developing transgenic tobacco seeds. The asglo5 gene promoter directed transcription in the endosperm as well as in the provascular tissue, the presumptive root tip and the shoot apical meristem of the embryo as revealed by GUS reporter gene constructs and in situ hybridization. However, immunological tissue printing detected the oat protein exclusively in the tobacco endosperm, suggesting that extensive post-transcriptional regulatory processes influence the expression of the monocot transgene in the dicot host.

