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Detection of 500-nucleotide DNA by laser desorption mass spectrometry
K Tang1, N I Taranenko, S L Allman
1Oak Ridge National Laboratory, Chemical Physics Section, Tennessee 37831-6378.
Rapid Communications in Mass Spectrometry : RCM
|September 1, 1994
Abstract:
We report the first detection of DNA segments as large as 500 nucleotides by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3-hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500-nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA-related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.