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Calpastatin gene in human testis

L F Wang1, S G Wei, S Y Miao

  • 1National Laboratory of Medical Molecular Biology, Beijing, China.

Biochemistry and Molecular Biology International
|May 1, 1994
PubMed
Summary

Antibodies from infertile women target a human sperm protein, BS-17. This protein is identified as the carboxyl terminus of calpastatin, a finding confirmed by antibody cross-reactivity.

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Area of Science:

  • Reproductive Biology
  • Molecular Biology
  • Immunology

Background:

  • Infertility can be associated with autoimmune responses targeting sperm components.
  • Identifying specific sperm antigens involved in infertility is crucial for understanding reproductive health.

Purpose of the Study:

  • To identify and characterize a specific glycoprotein (BS-17 component) from human sperm that interacts with antibodies found in infertile women's serum.
  • To determine the molecular identity and sequence of the BS-17 component.
  • To investigate the relationship between the BS-17 component and known proteins involved in reproductive function.

Main Methods:

  • Western blot analysis to detect antibody-antigen interactions.
  • Generation of polyclonal antibodies against the isolated BS-17 component.
  • Screening of a human testis expression library to identify positive clones.
  • Isolation and nucleotide sequencing of the complementary DNA (cDNA) encoding the BS-17 component.
  • Sequence homology analysis comparing BS-17 cDNA with known genes.
  • Immunological cross-reactivity testing with calpastatin.

Main Results:

  • Antibodies from infertile women's serum recognized a 17.5 kD glycoprotein (BS-17) in human sperm.
  • The cDNA sequence of BS-17 revealed homology with the 3' terminus of the calpastatin gene.
  • The BS-17 component corresponds to the carboxyl terminus of calpastatin, with 99.7% nucleotide and 99.5% amino acid identity.
  • Polyclonal antibodies against BS-17 also reacted with an 84 kD protein, consistent with the molecular weight of calpastatin.

Conclusions:

  • The BS-17 component of human sperm is identified as the carboxyl terminus of calpastatin.
  • This finding suggests a potential role for calpastatin in sperm function and possibly in infertility associated with autoimmune responses.

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