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Regeneration of yeast protoplasts. A freeze-etching study
Zeitschrift Fur Allgemeine Mikrobiologie
|January 1, 1976
Summary
Yeast protoplast regeneration involves structural changes in the plasma membrane and endoplasmic reticulum. Microfibrils forming the new cell wall do not interact with plasma membrane particles.
Area of Science:
- Cell biology
- Biochemistry
- Microscopy
Background:
- Yeast cell wall regeneration is crucial for cellular integrity.
- Understanding protoplast plasma membrane dynamics is key to cell wall synthesis.
Purpose of the Study:
- To investigate the submicroscopic structure of yeast protoplasts during cell wall regeneration.
- To determine the relationship between plasma membrane particles and newly synthesized microfibrils.
Main Methods:
- Freeze-etching electron microscopy was used to examine yeast protoplast structure.
- Protoplasts were cultured in liquid media and gelatine media to observe regeneration.
Main Results:
- No correlation was found between plasma membrane particle distribution and regeneration stage.
- Microfibrils did not penetrate the plasma membrane, indicating no direct structural link to membrane particles.
- Plasma membrane invaginations formed persistent furrows during regeneration, later breaking into shorter units.
- The endoplasmic reticulum was hypertrophic and showed parallel membrane arrangements.
Conclusions:
- Yeast cell wall microfibrils are synthesized independently of plasma membrane particles.
- Plasma membrane invaginations represent stable structures involved in regeneration.
- Endoplasmic reticulum proliferation supports active cell wall synthesis.