Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genomic imprinting. Imprinting with and without methylation

W Reik1, N D Allen

  • 1Laboratory of Developmental Genetics and Imprinting, AFRC Babraham Institute, Cambridge, U.K.

Current Biology : CB
|February 1, 1994
PubMed
Summary

DNA methylation is essential for maintaining parental imprinting in somatic cells. This process ensures genes are expressed based on their parental origin, as shown in methyltransferase-deficient mice.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cdk12 maintains the integrity of adult axons by suppressing actin remodeling.

Cell death discovery·2023
Same author

Integration of spatial and single-cell transcriptomic data elucidates mouse organogenesis.

Nature biotechnology·2021
Same author

SMAD transcription factors are altered in cell models of HD and regulate HTT expression.

Cellular signalling·2016
Same author

Integration-Free Reprogramming of Lamina Propria Progenitor Cells.

Journal of dental research·2016
Same author

Medical terminations of pregnancy: a viable source of tissue for cell replacement therapy for neurodegenerative disorders.

Cell transplantation·2011
Same author

Retinoid-independent motor neurogenesis from human embryonic stem cells reveals a medial columnar ground state.

Nature communications·2011

Area of Science:

  • Genetics
  • Epigenetics
  • Molecular Biology

Background:

  • Parental imprinting is an epigenetic phenomenon where genes are expressed in a parent-of-origin-specific manner.
  • The mechanisms maintaining imprinting throughout somatic cell divisions are not fully understood.

Purpose of the Study:

  • To investigate the role of DNA methylation in the somatic-cell maintenance of parental imprinting.
  • To determine if DNA methylation is a prerequisite for the stable inheritance of imprinting marks.

Main Methods:

  • Utilized methyltransferase-deficient mice models.
  • Analyzed gene expression patterns and DNA methylation status in somatic cells.

Main Results:

  • Methyltransferase deficiency disrupted the somatic-cell maintenance of parental imprinting.

Related Experiment Videos

  • Loss of DNA methylation led to altered gene expression, losing parent-of-origin specificity.

    • Conclusions:

    • DNA methylation is indispensable for the faithful somatic-cell propagation of parental imprinting.
    • This finding highlights the critical role of DNA methylation in epigenetic inheritance.