Related Experiment Videos
[Preliminary observation on the detection of schistosome circulating antigen and the immunocomplex by dot-ELISA]
Serum samples were collected from normal rabbits, normal persons, Schistosoma japonicum-infected rabbits and human cases respectively, and were treated by TCA precipitation and PEG precipitation, and subsequently detected by dot-ELISA. The results showed that both the supernatants of TCA-treated antisera from infected human cases and rabbits and the precipitants of PEG-treated antisera of the same origins showed positive reactions, while no positive reactions were found for sera from normal rabbits and normal persons. It was exhibited that dot-ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.
Serum samples were collected from normal rabbits, normal persons, Schistosoma japonicum-infected rabbits and human cases respectively, and were treated by TCA precipitation and PEG precipitation, and subsequently detected by dot-ELISA. The results showed that both the supernatants of TCA-treated antisera from infected human cases and rabbits and the precipitants of PEG-treated antisera of the same origins showed positive reactions, while no positive reactions were found for sera from normal rabbits and normal persons. It was exhibited that dot-ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.