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Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity
G C Machray1, L Burch, P E Hedley
1Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
FEBS Letters
|October 31, 1994
Abstract:
The cloning of a 1332 bp cDNA from a potato (Solanum tuberosum L.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.