Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A versatile method for efficient YAC transfer between any two strains

Y Hugerat1, F Spencer, D Zenvirth

  • 1Department of Genetics, Hebrew University, Jerusalem, Israel.

Genomics
|July 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Canadian Surgery Forum.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

Use of Nicergoline as Adjunctive Treatment of Neurotrophic Keratitis in Routine Clinical Practice: A Case Series.

Ocular immunology and inflammation·2021
Same author

Visual impairment induced by prosthetic cobaltism.

Archivos de la Sociedad Espanola de Oftalmologia·2021
Same author

Trends in corneal transplantation in a single center in Barcelona, Spain. Transitioning to DMEK.

Journal francais d'ophtalmologie·2019
Same author

Outbreak of invasive group A streptococcus: investigations using agar settle plates detect perineal shedding from a healthcare worker.

The Journal of hospital infection·2018
Same author

INDEPENDENT EVOLUTION OF A POLYGENIC SYSTEM IN ISOLATED POPULATIONS OF THE FUNGUS SCHIZOPHYLLUM COMMUNE.

Evolution; international journal of organic evolution·2017

Yeast artificial chromosome (YAC) transfer between yeast strains is now more versatile. A new method using kar1 intermediary strains enables efficient YAC manipulation for diverse research applications.

Area of Science:

  • * Molecular Biology
  • * Genetics
  • * Yeast Genetics

Background:

  • * Yeast artificial chromosomes (YACs) are crucial for cloning large DNA fragments.
  • * Efficient transfer of YACs between yeast hosts enhances experimental utility.
  • * Existing methods for YAC transfer can be limited in scope.

Purpose of the Study:

  • * To evaluate the general applicability of a new YAC transfer methodology.
  • * To assess YAC transfer efficiency using kar1 mutations in donor, recipient, or both yeast strains.
  • * To develop universal intermediary strains for flexible YAC transfer.

Main Methods:

  • * Utilizing kar1-mediated yeast mating for YAC transfer.
  • * Employing a set of four universal kar1 intermediary strains.

Related Experiment Videos

  • * Performing standard microbial manipulations (culture, replica plating) and pulsed-field gel electrophoresis (PFGE).

    • Main Results:

    • * Demonstrated successful YAC transfer across different kar1 mutant combinations.
    • * Developed four universal kar1 intermediary strains facilitating YAC transfer between any yeast strains.
    • * Verified YAC integrity and successful transfer using PFGE.

    Conclusions:

    • * Kar1-mediated YAC mating is an efficient, reliable, and flexible technique.
    • * The developed universal intermediary strains significantly simplify YAC manipulation.
    • * This methodology greatly facilitates diverse applications requiring YAC manipulation.