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Efficient gene transfer in Synechococcus sp. strains PCC 7942 and PCC 6301 by interspecies conjugation and
N F Tsinoremas1, A K Kutach, C A Strayer
1Department of Biology, Texas A&M University, College Station 77843-3258.
Journal of Bacteriology
|November 1, 1994
Abstract:
We developed a versatile, efficient genetic transfer method for Synechococcus sp. strains PCC 7942 and PCC 6301 that exceeds natural transformation efficiencies by orders of magnitude. As a test case, we complemented a histidine auxotroph and identified a hisS homolog of PCC 7942 as the complementing gene.