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Diagnostic approach to autoimmune blistering diseases using immunoblotting with chemiluminescence
T Kitamura1, Y Tamada, H Takama
1Department of Dermatology, Aichi Medical University, Japan.
The Journal of Dermatology
|September 1, 1994
Abstract:
Autoantibodies in sera from patients with autoimmune blistering diseases were detected by immunoblotting with chemiluminescence. This method provided the high sensitivity in the detection of autoantibodies against human epidermal extract. It was useful for detecting low titers of autoantibodies, and identifying small amounts of autoantigens in antigen extracts.