Modified E-rosette test for detection of total and active rosette-forming lymphocytes

The human peripheral blood T-lymphocyte population can be demonstrated with an economy of time by using brometlin-treated sheep red blood cells (SRBC). Similarity, the lower affinity, active T-cell population can be determined using intreated SRBC. Both tests are done without serum, or prolonged incubation of the cell mixture, using the same procedural steps reflecting changes as readily as longer test methods.