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[Clinical application of autoantibody idiotypes]
1Department of Clinical and Laboratory Medicine, Tohoku University School of Medicine, Sendai.
Idiotypes (Id) are unique antigenic determinants in hypervariable regions of immunoglobulins. This paper describes the clinical usefulness of Id for the determination of pathogenic autoantibodies. O-81-Id, human anti-DNA antibody idiotypes, were specifically detected in circulating and renal glomeruli-deposited immune complexes of patients with active lupus nephritis. Anti-idiotypic antibodies to anti-DNA antibodies could be also detected in human sera. Anti-Id antibodies to O-81-Id occurred spontaneously during the remission of systemic lupus erythematosus (SLE) but rarely during an acute episode. Monoclonal anti-Id antibodies had an ability to suppress in vitro anti-DNA antibody production by SLE lymphocytes. We also presented specific manipulation of anti-DNA production though the elimination of anti-DNA-Id-positive cells by using anti-Id-cytotoxic agent conjugates.
Idiotypes (Id) are unique antigenic determinants in hypervariable regions of immunoglobulins. This paper describes the clinical usefulness of Id for the determination of pathogenic autoantibodies. O-81-Id, human anti-DNA antibody idiotypes, were specifically detected in circulating and renal glomeruli-deposited immune complexes of patients with active lupus nephritis. Anti-idiotypic antibodies to anti-DNA antibodies could be also detected in human sera. Anti-Id antibodies to O-81-Id occurred spontaneously during the remission of systemic lupus erythematosus (SLE) but rarely during an acute episode. Monoclonal anti-Id antibodies had an ability to suppress in vitro anti-DNA antibody production by SLE lymphocytes. We also presented specific manipulation of anti-DNA production though the elimination of anti-DNA-Id-positive cells by using anti-Id-cytotoxic agent conjugates.