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Comparison of a PCR-based diagnostic assay for Mycoplasma pulmonis with traditional detection techniques
S Sanchez1, K Tyler, N Rozengurt
1Department of Veterinary Pathology, Royal Veterinary College, London, UK.
Laboratory Animals
|July 1, 1994
Abstract:
Current diagnosis of infection by Mycoplasma pulmonis, an important pathogen of laboratory rodent colonies worldwide, is based on serological, histopathological and culture techniques which can be slow and unreliable. A polymerase chain reaction (PCR) assay for M. pulmonis diagnosis was compared to current diagnostic methods. This PCR based technique allows a more specific, sensitive and rapid diagnosis of M. pulmonis from various tissues by comparison with culture and histopathology.