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Related Experiment Videos

Conductometric biosensor for ethanol detection based on whole yeast cells

Y I Korpan1, S V Dzyadevich, V P Zharova

  • 1Institute of Molecular Biology and Genetics, Academy of Science of Ukraine, Kiev.

Ukrainskii Biokhimicheskii Zhurnal (1978)
|January 1, 1994
PubMed
Summary

A novel yeast cell-based conductometric biosensor accurately quantifies ethanol in beverages. This microbial sensor offers rapid, stable, and reliable ethanol detection with a minimal detectable level of 1 mM.

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Area of Science:

  • Biosensor technology
  • Analytical chemistry
  • Biotechnology

Background:

  • Accurate ethanol quantification is crucial for alcoholic beverage quality control.
  • Existing methods like gas chromatography can be time-consuming and require specialized equipment.
  • Development of rapid, sensitive, and cost-effective biosensors is desirable.

Purpose of the Study:

  • To develop and characterize a yeast cell-based conductometric biosensor for ethanol quantification.
  • To evaluate the performance of the biosensor in terms of response time, linearity, sensitivity, and stability.
  • To compare the biosensor's results with those obtained by gas chromatography.

Main Methods:

  • Immobilization of yeast cells in Ca-alginate gel on gold planar electrodes.

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  • Measurement of conductivity changes upon specific ethanol consumption by yeast cells.
  • Computer-controlled sensor system for data acquisition.
  • Optimization of pH and buffer capacity for biosensor performance.

    • Main Results:

    • The biosensor exhibited a response time of less than 5 minutes.
    • Linearity was observed in the 5-100 mM ethanol concentration range on a logarithmic scale.
    • The minimal detectable ethanol level was 1 mM with a relative standard deviation of 10-12%.
    • Biosensor stability was enhanced at 4°C (up to 12 days) compared to room temperature (3 days).
    • Good correlation was found between biosensor results and gas chromatography.

    Conclusions:

    • The developed conductometric biosensor is a viable tool for rapid and accurate ethanol quantification in alcoholic beverages.
    • The biosensor demonstrates good sensitivity, linearity, and stability, particularly when stored at lower temperatures.
    • This technology offers a promising alternative to conventional methods for on-site or at-line ethanol analysis.