Related Experiment Video

Updated: Jul 22, 2026

Kupffer Cell Isolation for Nanoparticle Toxicity Testing
09:49

Kupffer Cell Isolation for Nanoparticle Toxicity Testing

Published on: August 18, 2015

[The role of Kupffer cells in regulating protein synthesis in hepatocytes]

L E Panin, I F Usynin, A V Khar'kovskiĭ

    Voprosy Meditsinskoi Khimii
    |July 1, 1994
    PubMed

    Keywords:
    liver cell co-culturenonparenchymal liver cellshepatocyte functionKupffer cell signaling

    More Related Videos

    Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method08:04

    Isolation of Primary Mouse Hepatocytes for Nascent Protein Synthesis Analysis by Non-radioactive L-azidohomoalanine Labeling Method

    Published on: October 23, 2018

    An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation05:42

    An Improved Time- and Labor- Efficient Protocol for Mouse Primary Hepatocyte Isolation

    Published on: October 25, 2021

    Related Concept Videos

    Cell Specific Gene Expression01:58

    Cell Specific Gene Expression

    Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
    Regulation of Nuclear Protein Sorting01:45

    Regulation of Nuclear Protein Sorting

    Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
    The Unfolded Protein Response01:37

    The Unfolded Protein Response

    The ER is the hub of protein synthesis in a cell. It has robust systems to quality control protein folding and also for degradation of terminally misfolded proteins. Under normal conditions, a small proportion of misfolded proteins that cannot be salvaged need to be transported to the cytoplasm by the ER-associated degradation or ERAD pathways. However, if the ERAD cannot handle the misfolded proteins, the cell activates the unfolded protein response or UPR to adjust the protein folding...
    Liver Regeneration01:24

    Liver Regeneration

    The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
    Cells of Liver
    The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are large...
    Regulation of Expression at Multiple Steps01:23

    Regulation of Expression at Multiple Steps

    The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
    Liver Physiology01:30

    Liver Physiology

    The liver, an essential organ in the human body, performs over 200 vital functions that can be broadly categorized into metabolic, hematological, endocrine regulation, and bile production.
    Metabolic Regulation:
    The liver is the central organ involved in regulating blood composition. It stabilizes blood glucose levels, maintaining them within the range of  70–110 mg/dL. When these levels drop, the liver breaks down glycogen reserves and releases glucose into the bloodstream. It can also...