Differential behavior of glial and neuronal cells exposed to hypotonic solution

The comparative modes of swelling of glial and neuronal cells were examined by video-enhanced differential interference contrast (VEC) microscopy. When exposed to hypotonic solution, C6 cells swelled slowly to 5 times their normal volume and burst, whereas N18 cells swelled more rapidly, forming blebs, and then burst partially. The time to burst was 410.6 +/- 45.7 s (mean +/- SD, n = 5) for C6, and 69.3 +/- 10.4 s (n = 5, p < 0.01) for N18, respectively. The present findings suggest a great difference in physical strength of the cell membrane between glial and neuronal cells: the former cell membrane appears to be elastic and tolerant to high tension, while the latter cell membrane is relatively weak.