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A method for activity staining of peroxidase and beta-1,3-glucanase isozymes in polyacrylamide electrophoresis gels
1Division of Plant Pathology, Newe Ya'ar Research Center, Haifa, Israel.
Analytical Biochemistry
|July 1, 1994
Abstract:
A procedure was developed for activity staining of peroxidase and beta-1,3-glucanase isozymes (a double staining procedure) in the same polyacrylamide gels using 3-amino-9-ethylcarbazol for peroxidase staining and laminarine and 2,3,5-triphenyltetrazolium chloride for glucanase staining. The procedure was used to detect peroxidase and beta-1,3-glucanase in different corn lines using plant leaf extract.