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Differential expression of cyclophilin isoforms during keratinocyte differentiation
D Chatellard-Gruaz1, J H Saurat, G Siegenthaler
1Clinique de Dermatologie, Hôpital Cantonal Universitaire, Genève, Switzerland.
The Biochemical Journal
|November 1, 1994
Summary
Cyclophilin A, a key protein in cyclosporin A binding, shows varied expression during human keratinocyte differentiation. Its isoforms are present in skin cells, with levels changing based on differentiation status.
Area of Science:
- Dermatology
- Biochemistry
- Cell Biology
Background:
- Cyclophilin A is the primary intracellular binding protein for cyclosporin A (CsA).
- Understanding its role in human keratinocyte differentiation is crucial for skin biology and CsA-related therapies.
Purpose of the Study:
- To investigate the presence and expression patterns of cyclophilin A and its isoforms in human keratinocytes during differentiation.
- To correlate cyclophilin expression with keratinocyte differentiation in vivo and in vitro.
Main Methods:
- Gel-filtration radiobinding-assay using tritiated CsA to analyze cyclophilin levels.
- PAGE radiobinding-assay on protein extracts from differentiating keratinocytes and human skin.
- Isoelectrofocusing autoradio-blotting-assay to detect cyclophilin isoforms.
Main Results:
- Gel-filtration showed one cyclophilin peak at 17 kDa, with levels not significantly differing during differentiation.
- PAGE revealed two CsA-binding peaks; a major peak was consistently expressed, while a minor peak decreased significantly in abnormal differentiation (psoriasis) and non-differentiated cells.
- Isoelectrofocusing detected multiple cyclophilin isoforms in normal skin, with some immunoreacting with cyclophilin antibodies.
Conclusions:
- Human epidermal cells contain several cyclophilin isoforms.
- The expression of these cyclophilin isoforms correlates with the differentiation state of human keratinocytes, both in vivo and in vitro.