Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid FISH technique for quantitative microscopy

F M Haar1, M Durm, K Aldinger

  • 1University of Heidelberg, FRG.

Biotechniques
|August 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The 4D Nucleome: Genome Compartmentalization in an Evolutionary Context.

Biochemistry. Biokhimiia·2018
Same author

In situ optical sequencing and structure analysis of a trinucleotide repeat genome region by localization microscopy after specific COMBO-FISH nano-probing.

Nanoscale·2015
Same author

Localization microscopy (SPDM) facilitates high precision control of lithographically produced nanostructures.

Micron (Oxford, England : 1993)·2014
Same author

Application perspectives of localization microscopy in virology.

Histochemistry and cell biology·2014
Same author

Functional nuclear organization of transcription and DNA replication: a topographical marriage between chromatin domains and the interchromatin compartment.

Cold Spring Harbor symposia on quantitative biology·2011
Same author

Analysis of Her2/neu membrane protein clusters in different types of breast cancer cells using localization microscopy.

Journal of microscopy·2010
Same journal

Investigating the interactomic landscape of survival motor neuron (SMN) and the SMNΔ7 truncated protein.

BioTechniques·2026
Same journal

Antigen retrieval-immunofluorescence on free floating sections to visualize the liver lobule and its cellular makeup.

BioTechniques·2026
Same journal

Special approach of droplet digital polymerase chain reaction (ddPCR) for transgene stability of a Chinese hamster ovary (CHO) cell line.

BioTechniques·2026
Same journal

Strand-specific quantification of L1 ORF0 and related transcripts by multiplex reverse transcription with tagged primers.

BioTechniques·2026
Same journal

Why and when should we choose digital PCR?

BioTechniques·2026
Same journal

Quantitative and unbiased lung alveolar septum assessment in an LPS experimental mouse model using 2D-spatial correlation image analysis from hematoxylin and eosin slides.

BioTechniques·2026
See all related articles

This study introduces a rapid, nonenzymatic fluorescence in situ hybridization (FISH) technique. This optimized FISH method accelerates signal detection and is suitable for quantitative microscopy applications.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cytogenetics

Background:

  • Fluorescence in situ hybridization (FISH) is a powerful cytogenetic technique.
  • Traditional FISH protocols can be time-consuming due to lengthy hybridization and multiple wash steps.

Purpose of the Study:

  • To develop and evaluate a rapid, nonenzymatic FISH technique.
  • To assess the suitability of the optimized technique for quantitative microscopy.

Main Methods:

  • Utilized repetitive DNA probes (pUC 1.77 and D15Z1) with fluorescein-12-dUTP labeling.
  • Employed a formamide-free buffer system for hybridization.
  • Reduced renaturation time to under 30 minutes and minimized wash steps to one.

Main Results:

Related Experiment Videos

  • Significantly reduced FISH procedure time.
  • Demonstrated successful differentiation of binding sites for pUC 1.77 probe based on fluorescence intensity and spot size.
  • Showed quantitative consistency in D15Z1 probe signal intensity across homologous chromosomes, with reduced variation at 30 min renaturation.

Conclusions:

  • The developed nonenzymatic FISH technique considerably accelerates the hybridization procedure.
  • The method is effective for quantitative microscopy, offering reliable signal detection.
  • This optimized FISH protocol enhances efficiency without compromising accuracy.