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Improved automatic kinetic method for the determination of various corticosteroids
L Ayllón1, M Silva, D Pérez-Bendito
1Department of Analytical Chemistry, Faculty of Sciences, University of Córdoba, Spain.
Journal of Pharmaceutical Sciences
|August 1, 1994
Summary
Developed new reaction-rate methods for corticosteroid determination, enhancing the Blue Tetrazolium and Porter-Silber reactions. The continuous-addition-of-reagent (CAR) technique improved accuracy and efficiency for micromolar corticosteroid analysis.
Area of Science:
- Analytical Chemistry
- Biochemistry
Background:
- Traditional Blue Tetrazolium and Porter-Silber reactions for corticosteroid analysis present challenges.
- These include high blank values, turbidity, and lengthy incubation periods at elevated temperatures.
Purpose of the Study:
- To develop improved reaction-rate methods for determining corticosteroids at micromolar concentrations.
- To adapt the Blue Tetrazolium and Porter-Silber reactions using the continuous-addition-of-reagent (CAR) technique.
Main Methods:
- Modified Blue Tetrazolium and Porter-Silber reactions were adapted for automatic kinetic determination.
- The continuous-addition-of-reagent (CAR) technique was employed to overcome traditional method limitations.
Main Results:
- The modified Blue Tetrazolium method demonstrated superior analytical figures of merit.
- This method enables corticosteroid determination from 2.7 x 10(-6) to 1.9 x 10(-4) M.
- Achieved a rate of 25 samples/h with a root mean square deviation of approximately 3%.
Conclusions:
- The developed Blue Tetrazolium method offers a robust and efficient approach for corticosteroid quantification.
- The CAR technique effectively addresses technical issues in traditional assays.
- The method's efficacy was validated through recovery experiments and analysis of commercial formulations.