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Neutrophil chemotaxis induced by immune complexes
A S Trevani1, P A Fontán, G A Andonegui
1Instituto de Investigaciones Hematológicas (IIHEMA), Academia Nacional de Medicina, Buenos Aires, Argentina.
Clinical Immunology and Immunopathology
|January 1, 1995
Summary
Soluble immune complexes (IC) induce neutrophil migration, but not IgG antibodies alone. This chemotaxis is mediated by Fc gamma receptors and can enhance formyl-methionyl-leucyl-phenylalanine (FMLP)-induced migration.
Area of Science:
- Immunology
- Cell Biology
Background:
- Neutrophils are critical immune cells involved in inflammatory responses.
- Immune complexes (IC) are formed by antibody-antigen binding and can activate immune cells.
- Fc gamma receptors on neutrophils mediate responses to IC.
Purpose of the Study:
- To investigate the ability of soluble immune complexes (IC) to induce neutrophil chemotaxis in vitro.
- To determine the role of Fc gamma receptors (FcγR) in IC-induced neutrophil migration.
- To assess the effect of IC on formyl-methionyl-leucyl-phenylalanine (FMLP)-induced neutrophil migration.
Main Methods:
- Preparation of soluble immune complexes (IC) using IgG antibodies.
- Neutrophil chemotaxis assays in vitro using checkerboard analysis.
- Inhibition studies using monoclonal antibodies (mAbs) against Fc gamma RII and Fc gamma RIII.
Main Results:
- Soluble IC induced neutrophil migration in a concentration-dependent manner.
- IgG antibodies alone did not induce neutrophil migration.
- IC-induced chemotaxis was significantly inhibited by anti-Fc gamma RII and Fc gamma RIII mAbs.
- IC enhanced FMLP-induced neutrophil migration in an additive manner.
Conclusions:
- Soluble IC are potent inducers of neutrophil chemotaxis.
- Fc gamma receptors, particularly Fc gamma RII and Fc gamma RIII, are crucial for IC-mediated neutrophil migration.
- IC can modulate neutrophil responses to other chemoattractants like FMLP.