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Updated: Aug 17, 2026
Preparation and Evaluation of 99mTc-labeled Tridentate Chelates for Pre-targeting Using Bioorthogonal Chemistry
Published on: February 4, 2017
Formulation of a single-component kit for the preparation of technetium-99m labelled ethyl cysteinate dimer:
A Afshan1, M Jehangir, M Ashraf
1Nuclear Chemistry Division, Pakistan Institute of Nuclear Science and Technology, Islamabad.
Abstract:
Ethyl cysteinate dimer (ECD) labelled with reduced technetium-99m has recently been proposed as a promising radiopharmaceutical for brain perfusion imaging. In the present study a single-component kit formulation has been developed, thus simplifying the radiolabelling procedure. A method of analysis by electrophoresis has also been developed, permitting identification of radiochemical impurities in the preparation. 99mTc-ECD prepared by the single-component kit was further evaluated in primates and humans. The results demonstrated that the complex is stable in vivo, rapidly extracted and retained in the brain tissue for a sufficient time for single-photon emission tomography studies. Therefore the present single-component kit formulation can be proposed as a reliable instant freeze-dried kit for routine preparation of 99mTc-ECD required for scintigraphic assessment of regional cerebral blood flow.
