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Sequences of heavy and light chain variable regions from four bovine immunoglobulins
K L Armour1, P R Tempest, P H Fawcett
1Scotgen Biopharmaceuticals Inc, Bridge of Don, Aberdeen, UK.
Molecular Immunology
|December 1, 1994
Abstract:
Oligodeoxyribonucleotide primers based on the 5' ends of bovine IgG1/2 and lambda constant (C) region genes, together with primers encoding conserved amino acids at the N-terminus of mature variable (V) regions from other species, have been used in cDNA and polymerase chain reactions (PCRs) to amplify heavy and light chain V region cDNA from bovine heterohybridomas. The amino acid sequences of VH and V lambda from four bovine immunoglobulins of different specificities are presented.