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Alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) applied to Dot-ELISA
A M Lissaldo1, S Hoshino-Shimizu, E S Umezawa
1Instituto de Ciências Biomédicas, USP, São Paulo, Brasil.
Revista Do Instituto De Medicina Tropical De Sao Paulo
|March 1, 1994
Abstract:
The alkaline soluble Trypanosoma cruzi epimastigote antigen (ASEA) was assessed in dot-ELISA for the diagnosis of Chagas' disease. Serum samples (355) from chagasic and non-chagasic patients were studied, and IgG antibodies to ASEA were found in all patients with chronic Chagas' disease. In non-chagasic patients 95.6% were negative, except for those with leishmaniasis (visceral and mucocutaneous), and some patients from control group reacted in low titers. The data indicate that dot-ELISA using ASEA is suitable for seroepidemiologic surveys to be employed in endemic areas for Chagas' disease.