[Histoimmunofluorescent demonstration of Br. suis in necropsy material]

Use was made of the direct immunofluorescence method for the demonstration of Brucella suis bacteria in paraffin and frozen histosections following the respective handling. The method was tested on necropsy material from experimentally infected test animals and spontaneously infected swine embryos. Compared with the cultural and serologic methods the histoimmunofluorescence one showed far better diagnostic possibilities. The use of the histoimmunofluorescence method is suggested along with the remaining routinely employed techniques in the laboratory practice. It will raise the effectiveness of the investigations for the demonstration of Brucella suis bacteria.